Cell Fusion Enhances Mesendodermal Differentiation of Human Induced Pluripotent Stem Cells.

ABSTRACT: Human induced pluripotent stem cells (iPS cells) resemble embryonic stem cells and can differentiate into cell derivatives of all three germ layers. However, frequently the differentiation efficiency of iPS cells into some lineages is rather poor. Here, we found that fusion of iPS cells with human hematopoietic stem cells (HSC) enhances iPS cell differentiation. Such iPS hybrids showed a prominent differentiation bias towards hematopoietic lineages but also towards other mesendodermal lineages. Additionally, during differentiation of iPS hybrids expression of early mesendodermal markers - Brachyury (T), MIX1 Homeobox-Like Protein 1 (MIXL1) and Goosecoid (GSC) - appeared with faster kinetics than in parental iPS cells. Following iPS hybrid differentiation there was a prominent induction of

ORGANISM(S): Homo sapiens

SUBMITTER: Martin Zenke 

PROVIDER: E-GEOD-58457 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress