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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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The microRNA-200 family regulates pancreatic beta-cell survival in type 2 diabetes (islet)

ABSTRACT: Islets from control and beta-cell specific miR-141/200c overexpressing mice were extracted and Affymetrix gene expression analysis performed We used Affymetrix Chip analysis to address which genes are regulated upon miR-200-overexpression in beta-cells at the age of 6 weeks. Transgenic mice overexpressing miR-141/200c and control mice were subjected to islet extraction, RNA was extracted using Trizol method, and Affymetrix analysis was performed.

ORGANISM(S): Mus musculus

SUBMITTER: Lennart Opitz

PROVIDER: E-GEOD-58488 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The microRNA-200 family regulates pancreatic beta-cell survival in type 2 diabetes (Min6 cells)

Project description:The microRNAs miR-141 and -200c were co-overexpressed in the Min6 beta-cell line, which caused pro-apoptotic gene expression and subsquently apoptosis. We used Affymetrix Chip analysis to address which genes are regulated upon miR-200-overexpression to define target genes of miR-200 and pro-apoptotic genes upregulated by miR-200 to induce apoptosis. Min6 cells were infected with a control Adenovirus or Adenovirus overexpression miR-141/200c. Cells were harvested with Trizol and RNA extraction was performed. RNA from n=3 wells was pooled for each condition and subjected to Affymetrix chip analysis

2015-05-06 | E-GEOD-58487 | biostudies-arrayexpress

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The microRNA-200 family regulates pancreatic beta-cell survival in type 2 diabetes

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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