Transcription profiling of human umbilical vein endothelial cells to identify targets of pharmacological histone deacetylase inhibition

ABSTRACT: We and others have previously reported the paradoxical downregulation of endothelial nitric oxide synthase (eNOS, NOS3) steady-state mRNA by pharmacological histone deacetylase (HDAC) inhibitors. This microarray experiment was designed to identify novel targets of an HDAC inhibitor (trichostatin A (TSA), 500 nM, 24h, Sigma) in primary human endothelial cells (human umbilical vein endothelial cells, HUVEC). Experiment Overall Design: HUVEC were isolated and cultured from three individuals (n=3, biological replicates). Paired samples were then treated with TSA (500nM, 24h, Sigma) or a vehicle-control. After sample preparation, hybridization to Affymetrix Human Genome Focus Arrays, image acquisition and the evaluation of several quality control metrics, summary expression measures were comput

ORGANISM(S): Homo sapiens

SUBMITTER: Philip Marsden 

PROVIDER: E-GEOD-5856 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress