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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Human MCF-7 breast cancer cells: control vs. treated with conditioned medium of cancer associated adipose tissue

ABSTRACT: Transcriptional profiling of human MCF-7 breast cancer cells comparing MCF-7 cells treated with control medium (DMEM/F12 + 0,5% BSA) with MCF-7 cells treated with conditioned medium of cancer-associated adipose tissue (CMCAAT) obtained from 2 breast cancer patients. Goal was to determine the effects of CMCAAT treatment on global MCF-7 gene expression. Two-condition experiment: MCF-7 control vs. CMCAAT treated cells. Biological replicates: 3 control replicates pooled as 1 (LL 1-2-4), 4 CMCAAT replicates (LL 6, LL 8, LL 9, LL 10)

ORGANISM(S): Homo sapiens

SUBMITTER: frédéric leprêtre

PROVIDER: E-GEOD-58574 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cancer-associated adipose tissue promotes breast cancer progression by paracrine oncostatin M and Jak/STAT3 signaling.

Lapeire Lore L Hendrix An A Lambein Kathleen K Van Bockstal Mieke M Braems Geert G Van Den Broecke Rudy R Limame Ridha R Mestdagh Pieter P Vandesompele Jo J Vanhove Christian C Maynard Dawn D Lehuédé Camille C Muller Catherine C Valet Philippe P Gespach Christian P CP Bracke Marc M Cocquyt Veronique V Denys Hannelore H De Wever Olivier O

Cancer research 20140924 23

Increasing evidence supports the critical roles played by adipose tissue in breast cancer progression. Yet, the mediators and mechanisms are poorly understood. Here, we show that breast cancer-associated adipose tissue from freshly isolated tumors promotes F-actin remodeling, cellular scattering, invasiveness, and spheroid reorganization of cultured breast cancer cells. A combination of techniques, including transcriptomics, proteomics, and kinomics enabled us to identify paracrine secretion of ...[more]

PMID: 25252914

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