Sphingosine-1-phosphate induces pro-remodelling response in airway smooth muscle cells

ABSTRACT: Background: Increased proliferation of airway smooth muscle (ASM) cells leading to hyperplasia and increased ASM mass is one of the most characteristic features of airway remodelling in asthma. A bioactive lipid, sphingosine-1-phosphate (S1P), has been suggested to affect airway remodelling by stimulation of human ASM cell proliferation. Objective: To investigate the effect of S1P on signalling and regulation of gene expression in ASM cells from healthy and asthmatic individuals. Methods: ASM cells grown from bronchial biopsies of healthy and asthmatic individuals were exposed to S1P. Gene expression was analysed using microarray, real-time PCR and western blotting. Receptor signalling and function was determined by mRNA knockdown and intracellular calcium mobilisation experiments. Results

ORGANISM(S): Homo sapiens

SUBMITTER: Grzegorz Woszczek 

PROVIDER: E-GEOD-58657 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress