A regulatory hierarchy controls the dynamic transcriptional response to extreme oxidative stress in archaea.

ABSTRACT: Previous work identified the winged helix-turn-helix DNA binding transcription factor (TF) RosR (encoded by VNG0258H gene), which dynamically regulates expression of more than 300 genes in response to oxidative stress in Halobacterium salinarum (Sharma 2012). RosR is required for survival of oxidants from multiple sources (e.g. H2O2 and paraquat), as deletion mutants are impaired for ROS outgrowth. Genes directly and indirectly controlled by RosR in response to ROS encode macromolecular repair functions. In the current study, we ask which of these genes are direct targets of RosR regulation. Dynamic chromatin immunoprecipitation combined with microarray (ChIP-chip) analysis validates that genes encoding these functions are direct targets of RosR binding and control. In addition, new RosR d

ORGANISM(S): Halobacterium sp. NRC-1

SUBMITTER: Amy Schmid 

PROVIDER: E-GEOD-58696 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress