Cell Context Dependent p53 Genome-Wide Binding Patterns and Enrichment at Repeats

ABSTRACT: We mapped the genomic binding sites of the tumor suppressor protein p53 in the human colorectal cancer cell line HCT116 and report here that the binding patterns of endogenous wild type p53 differed significantly between the genomes of the cancer cell line HCT116 and the normal human IMR90 fibroblasts (GSE31558) under the same experimental conditions (6 hr treatment with 5-fluorouracil). p53 binding differences affect promoter regions, CpG islands and major families of human repeat elements such as LTR, LINE and SINE. While p53 genomic binding sites residing in repeats have been reported before, we show here that the fraction of the p53 genomic binding sites residing in different repeat families differs between the normal and cancer human cell lines. We confirm that the p53 genomic binding

ORGANISM(S): Homo sapiens

SUBMITTER: Krassimira Botcheva 

PROVIDER: E-GEOD-58714 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress