Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

76 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

T cell responses to H1N1v and a longitudinal study of seasonal influenza vaccination - 2010

ABSTRACT: Systems biology approach to examine effects of seasonal flu vaccination in adults of different ages on gene expression, cytokine stimulation and serum cytokines with parameters such as immune senescence to uncover new markers and mechanisms behind failure of immune function in many older people. Total RNA was extracted from whole-blood lysates obtained from individuals immediately prior to vaccination against seasonal influenza, followed by depletion of globin messenger RNA. The goal was to evaluate potential gene expression signatures at Day 0 associated with immune senescence.

ORGANISM(S): Homo sapiens

SUBMITTER: David Furman

PROVIDER: E-GEOD-58898 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

T cell responses to H1N1v and a longitudinal study of seasonal influenza vaccination - 2009

Project description:Systems biology approach to examine effects of seasonal flu vaccination in adults of different ages on gene expression, cytokine stimulation and serum cytokines with parameters such as immune senescence to uncover new markers and mechanisms behind failure of immune function in many older people. Total RNA was extracted from whole-blood lysates obtained from individuals immediately prior to vaccination against seasonal influenza, followed by depletion of globin messenger RNA. The goal was to evaluate potential gene expression signatures at Day 0 associated with immune senescence.

2014-07-20 | E-GEOD-58943 | biostudies-arrayexpress

T cell responses to H1N1v and a longitudinal study of seasonal influenza vaccination - 2010

2014-07-20 | GSE58898 | GEO

T cell responses to H1N1v and a longitudinal study of seasonal influenza vaccination - 2011

2016-12-01 | GSE64343 | GEO

T cell responses to H1N1v and a longitudinal study of seasonal influenza vaccination - 2012

2014-12-25 | GSE64514 | GEO

T cell responses to H1N1v and a longitudinal study of seasonal influenza vaccination - 2009

2014-07-20 | GSE58943 | GEO

T cell responses to H1N1v and a longitudinal study of seasonal influenza vaccination - 2012

Project description:Systems biology approach to examine effects of seasonal flu vaccination in adults of different ages on gene expression, cytokine stimulation and serum cytokines with parameters such as immune senescence to uncover new markers and mechanisms behind failure of immune function in many older people. 1. Combinatorial tetramer analysis by FACS and/or CyTOF; Phosphoflow. 2. Luminex; Gene expression profiling (Affymetrix microarray). 3. Luminex; Gene expression profiling (Affymetrix microarry); Microneutralization assays. 4. Gene expression profiling (Affymetrix microarray); Immune phenotyping by CyTOF. 5. Gene expression profiling (Affymetrix microarray). 6. Immune phenotyping by CyTOF; Luminex. 7. ELISA.

2014-12-25 | E-GEOD-64514 | biostudies-arrayexpress

Baseline expressions in human whole blood

Project description:This study aims at identifying genes whose expression at baseline predicts the immune response to seasonal influenza vaccination. Blood samples were collected from healthy volunteers before their vaccination 91 Samples

2013-04-11 | E-GEOD-41080 | biostudies-arrayexpress

T cell responses to H1N1v and a longitudinal study of seasonal influenza vaccination

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-07-20 | E-GEOD-58970 | biostudies-arrayexpress

Predicting seasonal influenza vaccine response using systemic gene expression profiling

Project description:Seasonal influenza is a primary public health burden in the USA and globally. Annual vaccination programs are designed on the basis of circulating influenza viral strains. However, the effectiveness of the seasonal influenza vaccine is highly variable between seasons and among individuals. A number of factors are known to influence vaccination effectiveness including age, sex, and comorbidities. Here, we sought to determine whether whole blood gene expression profiling prior to vaccination is informative about pre-existing immunological status and the immunological response to vaccine. We performed whole transcriptome analysis using RNA sequencing (RNAseq) of whole blood samples obtained prior to vaccination from participants enrolled in an annual influenza vaccine trial. Serological status prior to vaccination and 28 days following vaccination were assessed using the hemagglutination inhibition assay (HAI) to define baseline immune status and the response to vaccination. We find evidence that genes with immunological functions are increased in expression in individuals with higher pre-existing immunity and in those individuals who mount a greater response to vaccination. Using a random forest model we find that this set of genes can be used to predict vaccine response with a performance similar to a model that incorporates physiological and prior vaccination status alone. Our study shows that increased expression of immunological genes, possibly reflecting greater plasmablast cell populations, prior to vaccination is associated with an enhanced response to vaccine. Furthermore, in the absence of demographic and physiological information gene expression signatures are informative about the likely response of an individual to seasonal influenza vaccination.

2022-07-11 | GSE207750 | GEO

Time Course of Adults Vaccinated with Influenza TIV Vaccine during 2010/11 Flu Season (FluCenter cohort)

Project description:Systems approaches have been used to describe molecular signatures driving immunity to influenza vaccination in humans. Whether such signatures are similar across multiple seasons, and in diverse populations is unknown. We applied systems approaches to study immune responses in young and, elderly subjects vaccinated with the seasonal influenza vaccine across 5 consecutive seasons. During the 2010 Influenza season, healthy adults were vaccinated with TIV, and blood samples isolated at days 0, 3, 7 post-vaccination. Microarrays were performed using total RNA extracted from the peripheral blood mononuclear cells of vaccinees.

2015-12-16 | E-GEOD-74815 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data