Acetylation of histone H4 at lysine 44 facilitates meiotic recombination by creating accessible chromatin [ChIP-seq]

ABSTRACT: Meiotic recombination hotspots are associated with histone post-translational modifications and open chromatin. However, it remains unclear how histone modifications and chromatin structure directly regulate meiotic recombination. Here, we identify acetylation of histone H4 at Lys44 (H4K44ac) as a new histone modification, occurring on the nucleosomal lateral surface. We show that H4K44ac is specific to yeast sporulation, rising during yeast meiosis and displaying genome-wide enrichment at recombination hotspots in meiosis. The H4K44 residue is required for normal meiotic recombination, for normal levels of double strand breaks during meiosis, and for optimal sporulation. Non-modifiable substitution H4K44R results in reduced MNase digestion and decreased binding of recombination-associated

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Gregory Donahue 

PROVIDER: E-GEOD-59003 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress