Early lineage restriction and regional segregation during mammalian heart development

ABSTRACT: Cardiac development arises from two sources of mesoderm progenitors, the first (FHF) and the second heart field (SHF). Mesp1 has been proposed to mark the most primitive multipotent cardiac progenitors common for both heart fields. Here, using clonal analysis of the earliest prospective cardiovascular progenitors in a temporally controlled manner during the early gastrulation, we found that Mesp1 progenitors consist of two temporally distinct pools of progenitors restricted to either the FHF or the SHF. FHF progenitors were unipotent, while SHF progenitors, were either uni- or bipotent. Microarray and single cell RT-PCR analysis of Mesp1 progenitors revealed the existence of molecularly distinct populations of Mesp1 progenitors, consistent with their lineage and regional contribution. Alto

ORGANISM(S): Mus musculus

SUBMITTER: Samira Chabab 

PROVIDER: E-GEOD-59033 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress