Expanding epigenomics to archived FFPE tissues: An evaluation of DNA repair methodologies

ABSTRACT: A study to evaluate techniques for repairing DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples by direct comparison of FFPE DNA repair methods prior to analysis on genome-wide methylation array to matched fresh frozen (FF) tissues. Formalin-fixed paraffin-embedded tissue from three colon adenocarcinoma samples were subjected to different FFPE-oriented DNA repair approaches and sequences, then compared with corresponding fresh frozen tissues. All samples were hybridized to the Illumina Infinium 450k Human Methylation BeadChip.

ORGANISM(S): Homo sapiens

SUBMITTER: Steven Eschrich 

PROVIDER: E-GEOD-59038 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress