Nucleosome occupancy changes in mammalian cell differentiation and reprogramming (microarray)

ABSTRACT: Embryonic stem cells (ESCs) and induced-pluripotent stem cells (iPSCs) self-renew and differentiate into an array of cell types in vitro and in vivo. A complex network of genetic and epigenetic pathways regulates the self-renewal and differentiation of these pluripotent cells, and the structure and covalent modifications of chromatin play a prominent role in this process. We examine nucleosome occupancy in mouse and human embryonic stem cells (ESCs), induced-pluripotent stem cells (iPSCs), and differentiated cell types using MNase-seq. To address variability inherent in this technique, we developed a bioinformatic approach that enabled the identification of regions of difference (RoD) in nucleosome occupancy between pluripotent and somatic cells. The majority of changes in nucleosomal sign

ORGANISM(S): Mus musculus

SUBMITTER: Robert Kingston 

PROVIDER: E-GEOD-59061 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress