Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Epigenomics of rat born to dams fed a diet deficient in methyl group donors (MDD)

ABSTRACT: It is clearly established that the maternal diet during pregnancy can induce physiological and metabolic adaptations in the developing fetus which determine its susceptibility later in life to develop diabetes, obesity... The molecular and genomic mechanisms underlying the programming of the metabolic syndrome remain largely unknown but may involve resetting of epigenetic marks and fetal gene expression. We analyzed the profile of the liver methylome in 21-day-old rats born to mothers fed with a standard diet or a diet lacking methyl donor nutrients (Vitamin B12 and folates) during gestation and lactation. Modifications of DNA methylation were found in the promoter regions of 1,032 genes out of 14,981 genes. Bioinformatics analysis revealed that these genes are mainly involved in glucose and lipid metabolism, nervous system, coagulation, endoplasmic reticulum (ER) stress and mitochondrial function. MDD induced modifications of methylation in rat liver were measured in 21-day-old rats born to dams fed with standard food or food deficient in methyl group donor. Six independent experiments were performed (3 controls versus 3 MDD).

ORGANISM(S): Rattus norvegicus

SUBMITTER: Sebastien Hergalant

PROVIDER: E-GEOD-59191 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Identification of master genes involved in liver key functions through transcriptomics and epigenomics of methyl donor deficiency in rat: relevance to nonalcoholic liver disease.

Chen Gaili G Broséus Julien J Hergalant Sébastien S Donnart Audrey A Chevalier Catherine C Bolaños-Jiménez Francisco F Guéant Jean-Louis JL Houlgatte Rémi R

Molecular nutrition & food research 20141209 2

<h4>Scope</h4>Our study aims to investigate molecular events associated to methyl donor deficiency (MDD) by analyzing the transcriptome and the methylome of MDD rats in liver.<h4>Methods and results</h4>Twenty-one-day-old rats born to mothers fed either with a standard diet or a MDD diet during gestation and lactation were compared. From a total of 44 000 probes for 26 456 genes, we found two gene clusters in MDD rats whose expression levels had significant differences compared with controls: 32 ...[more]

PMID: 25380481

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Dataset Information

Epigenomics of rat born to dams fed a diet deficient in methyl group donors (MDD)

Publications

Identification of master genes involved in liver key functions through transcriptomics and epigenomics of methyl donor deficiency in rat: relevance to nonalcoholic liver disease.

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