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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Genome wide expession analysis of effect of Batf2 knock down in bone marrow derived macrophage cells stimulated with IFNg

ABSTRACT: Bmdm cells were differentiated for 10 days and harvested and culture in six well plate followed by transfection with Batf2 ShRNA. Media was replanished in every two days and on 10th day cells were stimulated with IFNg for 4 hrs. Total RNA was obtain after 4 hrs of stimulation. Total RNA was colloected from control ShRNA transfected Bmdm cells and Batf2 shRNA transfected Bmdm cells stimulated with/with out IFNg

ORGANISM(S): Mus musculus

SUBMITTER: Harukazu Suzuki

PROVIDER: E-GEOD-59209 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome wide expession analysis of effect of Batf2 knock down in bone marrow derived macrophage cells stimulated with IFNg

Project description:Bmdm cells were differentiated for 10 days and harvested and culture in six well plate followed by transfection with Batf2 ShRNA. Media was replanished in every two days and on 10th day cells were stimulated with IFNg for 4 hrs. Total RNA was obtain after 4 hrs of stimulation.

2015-04-30 | GSE59209 | GEO

Genome wide expession analysis of mouse bone marrow derive macrophage (Bmdm) cell stimulated with IFNg

Project description:Bmdm cells were differentiated for 10 days and harvested and culture in six well plate followed by cytokine stimulation total RNA was harvested at 2, 4, 6, 24 hrs Total RNA was collected from bmdm cells stimulated with IFNg at different time point

2015-04-30 | E-GEOD-59207 | biostudies-arrayexpress

Genome wide expession analysis of mouse bone marrow derive macrophage (Bmdm) cell stimulated with cytokine and infected with mycobacterium tuberculosis

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Genome wide expession analysis of mouse bone marrow derive macrophage (Bmdm) cell stimulated with IFNg

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Genome wide expession analysis of mouse bone marrow derive macrophage (Bmdm) cell stimulated with cytokine and infected with mycobacterium tuberculosis

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Influences of CDK9 on gene expressions

Project description:To understand better the contribution of CDK9 in P. gingivalis-stimulated gene expression, gene chips were performed. Lentiviral vectors carrying CDK9 shRNA or not were transfected into cells for 96 hours, with the assist of Envirus. NC group was transfected with non-targeting scramble and stimulated with PBS. NC and Pg group was transfected with non-targeting scramble and stimulated with P. gingivalis. CDK9 shRNA group was transfected with CDK9 shRNA and stimulated with PBS. CDK9 shRNA and Pg group was transfected with CDK9 shRNA and stimulated with P. gingivalis.

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Alcohol-free fermented berry beverage phenolics attenuate diet-induced obesity and blood glucose in C57BL/6J mice

Project description:The objectives of this study were to understand the effect of phenolic compounds from fermented berry beverages on hyperglycemia and obesity in vivo using mice fed a high fat diet. Our hypothesis was that consumption of a fermented blueberry-blackberry beverage and its phenolic compounds would reduce the development of obesity and hyperglycemia in diet-induced obese mice. Body composition, histomorphological analysis of pancreatic islets and liver, and expression of genes involved in obesity and hyperglycemia were evaluated in order to explain the modulation of diet-induced obesity and hyperglycemia due to treatments. Total RNA was extracted from frozen pancreatic tissue of mice after 12 weeks of high-fat diet, 5 groups treated with sitagliptin, alcohol-free berry beverage (AFFB), 0.1X phenolic extract, 1X phenolic extract and 3X phenolic extract respectively, were compared to the control (water). Four replicates were included for each one of the treatments.

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Type I and II Interferon-Stimulated Genes in Murine Primary Bone Marrow Macrophages

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Nitric oxide engages an anti-inflammatory feedback loop mediated by peroxiredoxin 5 in phagocytes

Project description:Transcriptional profiling of murine dendritic cells stimulated with LPS and IFNg after shRNA knockdown of redox regulators.

2018-06-12 | GSE115553 | GEO

Genome wide expression analysis of bone marrow derived macrophage cells (BMDMs) stimulated with IFNg and effect of Batf2 knockdown in BMDMs stimulated with IFNg

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-04-30 | E-GEOD-59210 | biostudies-arrayexpress