Real-time quantitative PCR analysis of microRNAS in islets of mice exposed to low-protein diet during gestation

ABSTRACT: Maternal low-protein diet increases the susceptibility of offspring to type 2 diabetes. An insult to the pancreas during development can have long-term consequences on ?-cell mass and function. Because nutrients and growth factors signaling converge on mTOR, we hypothesized that mTOR plays a central role in ?-cell programming during fetal development. In this study, we revealed that newborns of dams exposed to low-protein diet (LP0.5) throughout pregnancy exhibited decreased insulin levels, ?-cell fraction, and mTOR signaling. Adult LP0.5 offspring demonstrated enhanced insulin sensitivity but remained glucose intolerant due to an insulin secretory defect and not reduced ?-cell mass. The insulin secretory defect was distal to Ca2+ influx, at the level of proinsulin biosynthesis and insulin

ORGANISM(S): Mus musculus

SUBMITTER: Emilyn Alejandro 

PROVIDER: E-GEOD-59279 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress