Translational profiling through biotinylation of tagged ribosomes in zebrafish

ABSTRACT: Phenotypic heterogeneity within a population of cells of the same cell type is a common theme in metazoan development. Apprehending complex developmental and physiological processes critically relies on our ability to probe the expression profile of these genetically defined cell sub-populations. Current strategies rely on cell enrichment based on tandem or simultaneous evaluation of multiple intersecting markers starting from a heterogeneous cellular suspension. The extensive tissue manipulations required for single-cell suspension generation, as well as the complexity of the required equipment, inherently complicates these approaches. Here, we propose an alternative methodology based on a genetically encoded system in the model organism Danio rerio (zebrafish). In transgenic fish, we tak

ORGANISM(S): Danio rerio

SUBMITTER: Michael Housley 

PROVIDER: E-GEOD-59355 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress