SCP-mediated chlorophyll synthesis and Photosystem II assembly maintains nitrogen homeostasis in Synechocystis sp. PCC 6803

ABSTRACT: Small CAB-like proteins (SCPs) are single-helix light-harvesting-like proteins found in all organisms performing oxygenic photosynthesis. We investigated the function of these stress-induced proteins in the cyanobacterium Synechocystis sp. PCC 6803 by comparing a strain, which expresses SCPs constitutively, with a mutant lacking all five SCPs. In the absence of SCPs, cells were larger, and showed irregular thylakoid structure and cell-surfaces. Deletion of scp genes strongly affected the carbon-nitrogen balance, causing accumulation of carbohydrates and a decrease in N-rich compounds (proteins and chlorophyll a). Data from transcriptomic and metabolomic experiments demonstrated that SCPs mediated stabilization of chlorophyll a under stress conditions is crucial to maintain the C/N balance.

ORGANISM(S): Synechocystis sp. PCC 6803

SUBMITTER: Miguel Hernández-Prieto 

PROVIDER: E-GEOD-59371 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress