Transcription profiling of differentiation of Rattus norvegicus oligodendrocytes from their progenitor cells

ABSTRACT: Oligodendrocytes undergo extensive changes as they differentiate from progenitors into myelinating cells. To better understand the; molecular mechanisms underlying this transformation, we performed a comparative analysis using gene expression profiling of A2B5+; oligodendrocyte progenitors and O4+ oligodendrocytes. Cells were sort-purified ex vivo from postnatal rat brain using flow cytometry. Using Affymetrix microarrays, 1707 transcripts were identified with a more than twofold increase in expression inO4+oligodendrocytes. Many genes required for oligodendrocyte differentiation were upregulated in O4+ oligodendrocytes, including numerous genes encoding; myelin proteins. Transcriptional changes included genes required for cell adhesion, actin cytoskeleton regulation, and fatty acid and; c

ORGANISM(S): Rattus norvegicus

SUBMITTER: Joe Nielsen 

PROVIDER: E-GEOD-5940 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress