Systematic Identification of Defined Conditions for Induction and Maintenance of Naive Human Pluripotency [expression]

ABSTRACT: Embryonic stem cells (ESCs) of mice and humans have distinct molecular and biological characteristics, raising the question whether an earlier ‘naive’ state of pluripotency may exist in humans. Here we took a systematic approach to identify small molecules that support autonomous self-renewal of naive human ESCs based on maintenance of endogenous OCT4 distal enhancer activity, a molecular signature of ground state pluripotency. Iterative chemical screening identified a combination of five kinase inhibitors that induces and maintains OCT4 distal enhancer activity when applied directly to conventional human ESCs. These inhibitors generate a homogeneous population of human pluripotent stem cells in which transcription factors associated with the ground state of pluripotency are highly upregul

ORGANISM(S): Homo sapiens

SUBMITTER: Rudolf Jaenisch 

PROVIDER: E-GEOD-59430 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress