Identification of apple miRNAs and their potentialrole in Fire Blight resistance

ABSTRACT: miRNAs are key players in multiple biological processes, therefore analysis and characterization of these small regulatory RNAs is a critical step towards better understanding of animal and plant biology. In apple (Malus domestica) two hundred microRNAs are known, which most probably represents only a fraction of miRNAome diversity. As a result, more effort is required to better annotate miRNAs and their functions in this economically important species. We performed deep sequencing of twelve small RNA libraries obtained for fire blight resistant and fire blight sensitive trees. In the sequencing results we identified 116 novel microRNAs and confirmed a majority of previously reported apple miRNAs. We then experimentally verified selected candidates with RT-PCR and stem-loop qPCR and perfo

ORGANISM(S): Malus domestica

SUBMITTER: El?bieta Kaja 

PROVIDER: E-GEOD-59482 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress