Asymmetric nucleosomes flank promoters in the budding yeast genome

ABSTRACT: Nucleosomes in active chromatin are dynamic, but whether they have distinct structural conformations is unknown. To identify nucleosomes with alternative structures genome-wide, we used H4S47C-anchored cleavage mapping, which revealed that nucleosomes at 5% of budding yeast nucleosome positions have asymmetric histone-DNA interactions. These asymmetric interactions are enriched at nucleosome positions that flank promoters. Micrococcal nuclease (MNase) sequence-based profiles of asymmetric nucleosome positions revealed a corresponding asymmetry in MNase protection near the dyad axis, suggesting that the loss of DNA contacts around H4S47 is accompanied by protection of the DNA from MNase. Chromatin immunoprecipitation mapping of selected nucleosome remodelers indicated that asymmetric nucleo

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jorja Henikoff 

PROVIDER: E-GEOD-59523 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress