Transformants of wheat GSK3/Shaggy-like kinase TaSK5 vs wild-type Col-0

ABSTRACT: A novel cold-inducible GSK3/Shaggy-like kinase cDNA (TaSK5) was isolated from winter wheat by a macroarray-based differential screening approach. Sequence analysis of TaSK5 revealed high similarity to Arabidopsis subgroup I GSK3/Shaggy-like kinases ASK-alpha, ASK-gamma and ASK-epsilon. Transgenic Arabidopsis plants overexpressing TaSK5 cDNA under the control of CaMV 35S promoter showed enhanced tolerance to salt and drought stresses. In contrast, the tolerance of the transgenic plants to freezing stress was not altered. To identify genes which are differentially regulated in the 35S:TaSK5 over-expressing Arabidopsis plants under non-stress conditions, we compared the genome-wide expression profiles of Col-0 and plants over-expressing TaSK5 using DNA microarrays. Sixty seven genes were foun

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Ryozo Imai 

PROVIDER: E-GEOD-59556 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress