Transcription profiling of human peripheral blood to detect cardiac allograft rejection and response to immunosuppressive therapy

ABSTRACT: BACKGROUND: Assessment of gene expression in peripheral blood may provide a noninvasive screening test for allograft rejection. We hypothesized that changes in peripheral blood expression profiles would correlate with biopsy-proven rejection and would resolve after treatment of rejection episodes. METHODS AND RESULTS: We performed a case-control study nested within a cohort of 189 cardiac transplant patients who had blood samples obtained during endomyocardial biopsy (EMB). Using Affymetrix HU133A microarrays, we analyzed whole-blood expression profiles from 3 groups: (1) control samples with negative EMB (n=7); (2) samples obtained during rejection (at least International Society for Heart and Lung Transplantation grade 3A; n=7); and (3) samples obtained after rejection, after treatment a

ORGANISM(S): Homo sapiens

SUBMITTER: Thomas Cappola 

PROVIDER: E-GEOD-5967 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress