Human somatic cell mutagenesis creates genetically tractable sarcomas

ABSTRACT: Creating spontaneous yet genetically tractable human tumors from normal cells presents a fundamental challenge. Retroviruses and transposons have been separately used as somatic cell insertional mutagens to identify cancer drivers in model organisms. Here we combined these mutagenic elements to enable cancer gene discovery starting with normal human cells. Lentivirus was used to seed gain- and loss-of-function gene disruption elements which were further deployed by Sleeping Beauty transposons throughout the genome of human bone explant mesenchymal cells. De novo tumors rapidly generated in this context were high-grade sarcomas corresponding to the spectrum of myxofibrosarcoma and undifferentiated pleomorphic sarcoma, aggressive neoplasms with a predilection for older adults. Tumor insertio

ORGANISM(S): Homo sapiens

SUBMITTER: Rama Khokha 

PROVIDER: E-GEOD-59720 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress