Escape from X inactivation in mouse tissues (RNA-Seq)

ABSTRACT: X chromosome inactivation (XCI) silences most genes on one X chromosome in female mammals, but some genes escape XCI. To identify escape gene in vivo and to explore molecular mechanisms that regulate this process we analyzed the allele-specific expression and chromatin structure of X-linked genes in mouse tissues and cells with skewed XCI and distinguishable alleles based on single nucleotide polymorphisms. Using a new method to estimate allelic expression, we demonstrate a continuum between complete silencing and significant expression from the inactive X (Xi). Few genes (2-3%) escape XCI to a significant level and only a minority differs between mouse tissues, suggesting stringent silencing and escape controls. Allelic profiles of DNase I hypersensitivity and RNA polymerase II occupancy

ORGANISM(S): Mus musculus x Mus spretus

SUBMITTER: Xinxian Deng 

PROVIDER: E-GEOD-59777 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress