Differential gene expression in mice liver following bulaquine administration

ABSTRACT: Global gene expression in livers of bulaquine treated mice was compared with control mice at different time points. Keywords: Time course Mice (Mus musculus) belonging to control and bulaquine-administered groups (n=4) were sacrificed. The livers were snap frozen in liquid nitrogen, and subsequently stored at -80ºC till further use. Liver samples were homogenized and total RNA was extracted with TRI reagent. 25 ug of total RNA was converted into labeled cDNA using CyScribe first strand cDNA labeling kit. The Cy5 and Cy3 labeled cDNAs were resuspended in Cyscribe Hyb buffer containing 10ug/ml sheared Salmon sperm DNA and 10ug/ml Yeast tRNA. The labeled samples were hybridized to the arrays and incubated for 16-18hrs at 42ºC. Fluorescent array images were collected for both Cy3 and Cy5 wit

ORGANISM(S): Mus musculus

SUBMITTER: Srikanta Rath 

PROVIDER: E-GEOD-5980 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress