Global Identification of Protein PTMs in a Single-pass Database Search

ABSTRACT: Bottom-up proteomics database search algorithms used for peptide identification cannot comprehensively identify posttranslational modifications (PTMs) in a single-pass because of high false discovery rates (FDRs). A new approach to database searching enables Global PTM (G-PTM) identification by exclusively looking for curated PTMs, thereby avoiding the FDR penalty experienced during conventional variable modification searches. We identified nearly 2500 unique, high-confidence modified peptides comprising 31 different PTM types in single-pass database searches. Male C57BL/6J (B6) and CAST/EiJ (CAST) mice were purchased from The Jackson Laboratories (Bar Harbor, Maine) and housed in an environmentally controlled vivarium at the University of Wisconsin Biochemistry Department. Mice were provi

ORGANISM(S): Mus musculus

SUBMITTER: Anthony Cesnik 

PROVIDER: E-GEOD-59956 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress