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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide miRNA turnover profile after transcription inhibition in HDMYZ cells with biological triplicate

ABSTRACT: HDMYZ cells were treated with 4ug/ml ActD for 0, 1, 4 and 12 hours. Small RNAs of 15-40 bases were gel-purified from 5 ug total RNA, and subjected to multiplex Illumina small RNA library preparation. Small RNA libraries were sequenced on a HiSeq2000 (Illumina). Measure miRNA expression level at 0, 1, 4, 12 hours post ActinomycinD treatment to examine miRNA turnover pattern.

ORGANISM(S): Homo sapiens

SUBMITTER: Mei Zhong

PROVIDER: E-GEOD-60036 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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