Transcriptome-wide mapping reveals widespread dynamic regulated pseudouridylation of mRNA

ABSTRACT: Pseudouridine is the most abundant modification occurring on RNA, yet with the exception of a few well-studied RNA molecules little is known about the modified positions and their function(s). Here, we develop M-NM-(-seq, a method for transcriptome-wide quantitative mapping of pseudouridine. We validate M-NM-(-seq with synthetic spike-ins and de novo identification of the vast majority of previously reported pseudouridylated positions. M-NM-(-seq permits discovery of hundreds of novel pseudouridine modifications in human and yeast mRNAs and snoRNAs. Knockdown and knockout of pseudouridine synthases uncovers the cognate PUSs mediating pseudouridine catalysis at these individual novel sites and their target sequence features. In both human and yeast pseudouridine formation on mRNA depends on

ORGANISM(S): Candida albicans

SUBMITTER: Schraga Schwartz 

PROVIDER: E-GEOD-60047 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress