Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Expression data from Brakeless mutant Drosophila embryos

ABSTRACT: The Brakeless protein performs many important functions during Drosophila development, but how it controls gene expression is not understood. We previously showed that Brakeless can function as a transcriptional co-repressor. Here, we report transcriptional profiling of brakeless mutant embryos to identify additional target genes. We used microarrays to identify gene expression changes in brakeless mutant early Drosophila embryos. Drosophila germline clones of the strong brakeless 278 allele (lab stock B97) were generated and compared to control germline clones generated with the original unmutagenized chromosome (FRT G13). Two-four hour old embryos from these females were used for RNA extraction and hybridization on Affymetrix microarrays.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Mattias Mannervik

PROVIDER: E-GEOD-60048 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The Brakeless co-regulator can directly activate and repress transcription in early Drosophila embryos.

Crona Filip F Holmqvist Per-Henrik PH Tang Min M Singla Bhumica B Vakifahmetoglu-Norberg Helin H Fantur Katrin K Mannervik Mattias M

Developmental biology 20150807 1

The Brakeless protein performs many important functions during Drosophila development, but how it controls gene expression is poorly understood. We previously showed that Brakeless can function as a transcriptional co-repressor. In this work, we perform transcriptional profiling of brakeless mutant embryos. Unexpectedly, the majority of affected genes are down-regulated in brakeless mutants. We demonstrate that genomic regions in close proximity to some of these genes are occupied by Brakeless, ...[more]

PMID: 26260775

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Project description:DRM is a conserved transcription factor complex that includes E2F/DP and pRB family proteins and plays important roles in development and cancer. Here we perform microarray expression profiling analysis of lin-54, a DNA-binding member of the DRM complex. To identify genes regulated by LIN-54 in soma and germline, we analyzed wild-type and lin-54 mutant C. elegans embryos and isolated germlines. We chose embryos because they consist primarily of somatic cells, at a developmental stage with both active cell divisions and dynamic developmental gene expression programs. Since lin-54 null animals are sterile, embryos were obtained from a strain carrying the partial loss-of-function allele lin-54(n2990). Germlines were dissected from lin-54(n3423) null adults that lack detectable transcript and protein. The results revealed conserved roles for DRM in regulating genes involved in cell division, development, and reproduction. We find LIN-54 promotes expression of reproduction genes in the germline, but prevents ectopic activation of germline-specific genes in embryonic soma. Strikingly, genomics and cytological analyses show that DRM binding, a DRM binding motif, and LIN-54-regulated genes are all autosome-enriched. One paradoxical exception occurs the germline, where DRM binds autosomes but genes down-regulated in DRM mutants are enriched on X chromosomes. We compared embryonic or germline gene expression profile of lin-54 mutants with that of wild-type N2 C. elegans. Embryos were obtained from a strain carrying the partial loss-of-function allele lin-54(n2990) grown at 25C for one generation. Germlines were isolated from lin-54(n3423) null adults that lack detectable lin-54 transcript and protein. We isolated the germline region from the tip until late pachytene stage of meiosis, because nuclei in this region are morphologically similar between wild-type and mutant and are all undergoing X chromosome silencing. 3 biological replicates of each genotype/tissue were examined.

Dataset Information

Expression data from Brakeless mutant Drosophila embryos

Publications

The Brakeless co-regulator can directly activate and repress transcription in early Drosophila embryos.

Similar Datasets

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