SiRNA profiling of human embryonic fibroblasts transfected with mock, EZH2, EED, SUZ12, and BMI-1 siRNA reveals genome-wide mapping of Polycomb target genes unravels their roles in cell fate transitions

ABSTRACT: The Polycomb group (PcG) proteins form chromatin-modifying complexes that are essential for embryonic development and stem cell renewal and are commonly deregulated in cancer. Here, we identify their target genes using genome-wide location analysis in human embryonic fibroblasts. We find that Polycomb-Repressive Complex 1 (PRC1), PRC2, and tri-methylated histone H3K27 co-occupy >1000 silenced genes with a strong functional bias for embryonic development and cell fate decisions. We functionally identify 40 genes derepressed in human embryonic fibroblasts depleted of the PRC2 components (EZH2, EED, SUZ12) and the PRC1 component, BMI-1. Interestingly, several markers of osteogenesis, adipogenesis, and chrondrogenesis are among these genes, consistent with the mesenchymal origin of fibroblasts

ORGANISM(S): Homo sapiens

SUBMITTER: Adrian Bracken 

PROVIDER: E-GEOD-6015 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress