Chromatin immunoprecipitation of HeLa cells after immunoprecipitation of U2AF65 associated mRNAs

ABSTRACT: U2AF65 is an essential splicing factor involved in the 3'splice site recognition dureing the first steps of spliceosome assembly. In addition, this protein has nucleocytoplasmic shuttling activity and the Drosophila homologue has been implicated in mRNA export. We have used microarray hybridization coupled to RNA immunoprecipitation from HeLa cell cytoplasmic extracts using anti-U2AF65 antibodies to identify mRNA molecules associated with the U2AF65 splicing factor; Sample preparation methods (Gama-Carvalho et al, 2006, submitted); Suspension HeLa cells were grown in DMEM, 10% FCS, Pen/Strep and split 1:2 the day before harvesting. Post-nuclear cytoplasmic extracts were obtained as described (Herbert and Hecht 1999), using 5x107 cells/ml of lysis buffer. mRNAs were isolated by immunoprecip

ORGANISM(S): Homo sapiens

SUBMITTER: Margarida Gama-Carvalho 

PROVIDER: E-GEOD-6022 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress