Enhancer Activation Requires Trans-Recruitment of a Mega Transcription Factor Complex (ChIP-seq)

ABSTRACT: Enhancers provide critical information directing cell-type specific transcriptional programs, regulated by binding of signal-dependent transcription factors and their associated cofactors. Here we report that the most strongly activated estrogen (E2)-responsive enhancers are characterized by trans-recruitment and in situ assembly of a large 1-2MDa complex of diverse DNA-binding transcription factors by ERα at ERE-containing enhancers. We refer to enhancers recruiting these factors as mega transcription factor-bound in trans (MegaTrans) enhancers. The MegaTrans complex is a signature of the most potent functional enhancers and is required for activation of enhancer RNA transcription and recruitment of coactivators, including p300 and Med1. The MegaTrans complex functions, in part, by recru

ORGANISM(S): Homo sapiens

SUBMITTER: Daria Merkurjev 

PROVIDER: E-GEOD-60270 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress