Interaction between TBP and condensing drives the organization and faithful segregation of mitotic chromosomes

ABSTRACT: Genome/chromosome organization is highly ordered and controls nuclear events. Here, we show that the TATA box-binding protein (TBP) interacts with the Cnd2 kleisin subunit of condensin to mediate interphase and mitotic chromosome organization in fission yeast. TBP recruits condensin onto RNA polymerase III-transcribed (Pol III) genes and highly transcribed Pol II genes; condensin in turn associates these genes with centromeres. Inhibition of the Cnd2-TBP interaction disrupts condensin localization across the genome and the proper assembly of mitotic chromosomes, leading to severe defects in chromosome segregation and eventually causing cellular lethality. We propose that the Cnd2-TBP interaction coordinates transcription with chromosomal architecture by linking dispersed gene loci with cen

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Hideki Tanizawa 

PROVIDER: E-GEOD-60273 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress