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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Impact of E2 ubiquitin conjugating activity of MUC1 on gene expression

ABSTRACT: We identified a novel E2 ubiquitin conjugating activity within the C-terminus domain of MUC1. To determine if E2 ubiquitin conjugating activity of MUC1 is responsible for regulating expression of oncogenic genes we isolated RNA from vector control cells, MUC1 wildtype overexpression cells, and cells that express a serine mutant lacking E2 activity. RNA was isolated from each of the 3 cell lines, S2013.Neo, S2013.MUC1-WT, and S2013.MUC1-Ser. Gene expression was analyzed by spotted microarray and the differences in gene expression were calculated between the cell lines

ORGANISM(S): Homo sapiens

SUBMITTER: Ryan Hanson

PROVIDER: E-GEOD-60339 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Impact of E2 ubiquitin conjugating activity of MUC1 on gene expression

Project description:We identified a novel E2 ubiquitin conjugating activity within the C-terminus domain of MUC1. To determine if E2 ubiquitin conjugating activity of MUC1 is responsible for regulating expression of oncogenic genes we isolated RNA from vector control cells, MUC1 wildtype overexpression cells, and cells that express a serine mutant lacking E2 activity.

2016-08-01 | GSE60339 | GEO

MicroRNAs differentially expressed in MUC1 overexpressing cells

Project description:To determine if overexpression of MUC1 alters the microRNA profile of pancreatic cancer cells S2.013. Comparison of miRNAs differentially regulated in pancreatic cancer cell line S2.013 with and without MUC1 overexpression. MiRCURY LNA microarray was used to determine differential microRNA expression in one replicate of S2.013.Neo (control) cells compared to S2.013.MUC1 (experimental) cells. Confirmation with qRT-PCR was performed on a subset of microRNAs identified as differentially regulated between the two groups.

2013-08-10 | E-GEOD-48185 | biostudies-arrayexpress

Transcription profiling of rat 3Y1 embryonic fibroblasts to identify MUC1-induced transcriptional alterations

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2009-04-19 | E-GEOD-14337 | biostudies-arrayexpress

Expression of genes in the spleen of WT and MUC1.Tg mice immunized with dendritic cells loaded with MUC1 peptide

Project description:Goal of study: To determine differentially expressed genes with potential roles in tolerance and immunity in MUC1-immunized WT and MUC1.Tg mice WT (C57Bl/6) and MUC1.Tg mice were immunized (i.v.) with dendritic cells loaded with sythetic, human MUC1 peptide. At 24h and 72h splenic RNA from vaccination groups (n=3) was pooled and microarray performed.

2013-01-16 | E-GEOD-43503 | biostudies-arrayexpress

Human renal clear-cell carcinoma cells : control vs MUC1 overexpressing cells

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MUC1(insC) affects vesicular transport in renal epithelial cells

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Effect of Rad6 backside beta-turn mutations on the chromatin occupancy of the histone H2B ubiquitin-conjugating complex subunits

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2022-10-24 | GSE139633 | GEO

Interactome of E1 ubiquitin-activating enzymes and of E2 ubiquitin-conjugating enzymes in human HEK293 cells.

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2023-11-14 | PXD042361 | Pride

Identification of differential gene expression modulated by knockdown of MUC1 in MKN45 gastric carcinoma cells

Project description:Transcripts upregulated or downregulated by knockdown of MUC1 were identified through expression profiling of a total of 12,135 genes in comparison with MKN45- MUC1 RNAi clones and control clones. We endeavored to identify genes which expression is affected by MUC1 by performing cDNA microarray analysis on two MKN45 MUC1 RNAi clones and one control clone.

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Functional conservation and divergence of the helix-turn-helix motif of E2 ubiquitin-conjugating enzymes

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