Model-driven mapping of transcriptional networks reveals the circuitry and dynamics of virulence regulation

ABSTRACT: Purpose: Key steps in understanding a biological process include identifying genes that are involved and determining how they are regulated. We developed a novel method for identifying TFs involved in a specific process and used it to map regulation of the key virulence factor of Cryptococcus neoformans, its capsule. Results: The map, built from expression profiles of 41 TF mutants, includes 20 TFs not previously known to regulate virulence attributes. It also reveals a hierarchy comprising executive, mid-level, and “foreman” TFs. When grouped by temporal expression pattern, these TFs explain much of the transcriptional dynamics of capsule induction. Phenotypic analysis of 41 TF deletion mutants revealed complex relationships among virulence factors and virulence in mice. Conclusions: Thes

ORGANISM(S): Cryptococcus neoformans

SUBMITTER: Ezekiel Maier 

PROVIDER: E-GEOD-60398 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress