Identification of RNAs associated with telomeres by enChIP-RNA-Seq

ABSTRACT: Accumulating evidence suggests important roles of RNAs interacting with genomic regions in the regulation of genome functions including X chromosome inactivation and gene expression. However, no method to identify RNAs interacting with a given genomic region in a non-biased manner has been reported. Here, we used engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) combined with the RNA-Seq analysis (enChIP-RNA-Seq) to perform non-biased search of RNAs interacting with telomeres. In enChIP-RNA-Seq, the target genomic regions are captured with an engineered DNA-binding molecule such as a TAL protein. Subsequently, RNAs are purified and subjected to the RNA-Seq analysis. The detected RNAs contained known telomere-binding RNAs including telomerase RNA and Cajal body

ORGANISM(S): Mus musculus

SUBMITTER: Daisuke Okuzaki 

PROVIDER: E-GEOD-60425 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress