Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse embryonic fibroblasts derived from the Dicer knockout

ABSTRACT: A functional part of the Dicer gene was knocked out from MEF using a conditional knockout strain; Expression of miRNA target genes were evaluated to gain better understanding of regulation by miRNAs Experiment Overall Design: MEF_CDKO+Tamoxifen (2 concentrations and 2 replicates each = 4 arrays) were compared to MEF_CDKO No Treatment / MEF_WT+Tamoxifen / MEF_WT No Treatment (2 replicates each = 6 arrays)

ORGANISM(S): Mus musculus

SUBMITTER: Noam Shomron 

PROVIDER: E-GEOD-6046 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Determinants of targeting by endogenous and exogenous microRNAs and siRNAs.

Nielsen Cydney B CB   Shomron Noam N   Sandberg Rickard R   Hornstein Eran E   Kitzman Jacob J   Burge Christopher B CB  

RNA (New York, N.Y.) 20070913 11

Vertebrate mRNAs are frequently targeted for post-transcriptional repression by microRNAs (miRNAs) through mechanisms involving pairing of 3' UTR seed matches to bases at the 5' end of miRNAs. Through analysis of expression array data following miRNA or siRNA overexpression or inhibition, we found that mRNA fold change increases multiplicatively (i.e., log-additively) with seed match count and that a single 8 mer seed match mediates down-regulation comparable to two 7 mer seed matches. We identi  ...[more]

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