IRNA-seq: Computational method for genome wide assessment of acute transcriptional regulation from total RNA-seq data

ABSTRACT: RNA-seq is a sensitive and accurate technique to compare steady state levels of RNA between different cellular states. However, as it does not provide an account of transcriptional activity per se, other technologies are needed to more precisely determine acute transcriptional responses. Here, we have developed an easy, sensitive and accurate novel method, iRNA-seq, for genome-wide assessment of transcriptional activity based on analysis of intron coverage from total RNA-seq data. To test our method, we have performed total RNA-seq and RNA polymerase II (RNAPII) ChIP-seq profiling of the acute transcriptional response of human adipocytes to TNFM-NM-1 treatment and analyzed these using iRNA-seq in addition to different publically availbale dataset. Comparison of the results derived from iR

ORGANISM(S): Homo sapiens

SUBMITTER: Susanne Mandrup 

PROVIDER: E-GEOD-60462 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress