Disruption of transkingdom communication by antibiotics leads to immune and mitochondrial dysfunction in the gut

ABSTRACT: We analyzed the effects of antibiotics using a popular model of gut microbiota depletion in mice by a cocktail of antibiotics. We combined intestinal transcriptome together with metagenomic analysis of the gut microbiota to develop a new bioinformatics approach that probes the links between microbial components and host functions. We found that most antibiotic-induced alterations can be explained by three factors: depletion of the microbiota; direct effects of antibiotics on host tissues; and the effects of remaining antibiotic-resistant microbes. While microbe depletion led to down-regulation of immunity, the two other factors primarily inhibited mitochondrial gene expression and amounts of active mitochondria, and induced cell death. By reconstructing and analyzing a transkingdom network

ORGANISM(S): Mus musculus

SUBMITTER: Natalia Shulzhenko 

PROVIDER: E-GEOD-60568 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress