Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

An alternative pluripotent state confers interspecies chimaeric competency [RNA-Seq]

ABSTRACT: Here we show that by simple modulation of extrinsic signaling pathways, a new class of pluripotent stem cells, referred to as region selective epiblast stem cells (rsEpiSCs), could be efficiently derived from different stages of the early embryo. rsEpiSCs share features of primed pluripotency yet are distinct from EpiSCs in their molecular characteristics and ability to colonize post-implantation embryos. We performed RNA-sequencing experiments and examined the global gene expression profiles of EpiSCs, rsEpiSCs, in vivo isolated four regions of E6.5 mouse epiblasts: AP (anterior-proximal), AD (anterior-distal), PP (posterior-proximal) and PD (posterior-distal), human H1 ESCs, H1 rsESCs, H9 ESCs, H9 rsESCs, rhesus monkey ORMES23 rsESCs, and chimpanzee rsiPSCs. Examination of global gene expression profiles in 2 pluripotent stem cell types across multiple species.

ORGANISM(S): Pan troglodytes

SUBMITTER: Christopher Benner 

PROVIDER: E-GEOD-60603 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Pluripotency, the ability to generate any cell type of the body, is an evanescent attribute of embryonic cells. Transitory pluripotent cells can be captured at different time points during embryogenesis and maintained as embryonic stem cells or epiblast stem cells in culture. Since ontogenesis is a dynamic process in both space and time, it seems counterintuitive that these two temporal states represent the full spectrum of organismal pluripotency. Here we show that by modulating culture paramet  ...[more]

Similar Datasets

Genomics An alternative pluripotent state confers interspecies chimaeric competency [ChIP-seq]
2015-05-06 | E-GEOD-60604 | biostudies-arrayexpress
Transcriptomics An alternative pluripotent state confers interspecies chimaeric competency [Microarray]
2015-05-06 | E-GEOD-60523 | biostudies-arrayexpress
Genomics Nucleoporin Nup153 Regulates Stem Cell Pluripotency through Gene Silencing
2015-06-19 | E-GEOD-64008 | biostudies-arrayexpress
Transcriptomics The DAXX co-repressor is directly recruited to active regulatory elements genome-wide to regulate autophagy programs in a model of human prostate cancer (RNA-seq)
2015-06-24 | E-GEOD-68645 | biostudies-arrayexpress
Transcriptomics Transcriptomic changes in Drosophila tissues under Time-restricted Feeding (TRF)
2015-02-18 | E-GEOD-64108 | biostudies-arrayexpress
Transcriptomics Conserved non-coding sequence 2 (CNS2) of the Foxp3 genomic locus is essential for the function and stability of regulatory T cells during their activation
2014-09-04 | E-GEOD-57272 | biostudies-arrayexpress
Transcriptomics miR-23~27~24 clusters control effector T cell differentiation and function
2015-12-11 | E-GEOD-75909 | biostudies-arrayexpress
Transcriptomics Modeling human brain evolution using induced pluripotent stem cells: comparative analysis of neuronal development in humans and chimpanzees
2016-08-07 | E-GEOD-83638 | biostudies-arrayexpress
Transcriptomics Transcriptomic analysis reveals novel long non-coding RNAs critical for vertebrate development [RNA-Seq]
2015-05-15 | E-GEOD-54968 | biostudies-arrayexpress
Genomics A pseudogene lncRNA at the interface of inflammation and anti-inflammatory therapeutics
2013-08-05 | E-GEOD-47494 | biostudies-arrayexpress