Identification of circulating miRNAs in a mouse model of nerve allograft transplantation under FK506 immunosuppression by Illumina small RNA deep sequencing

ABSTRACT: This study aimed to establish the expression profile of circulating microRNAs (miRNAs) during nerve allotransplantation. Experimental design Male Balb/c and C57BL/6 mice (age, 10M-bM-^@M-^S12 weeks; weight, 30M-bM-^@M-^S35 g) were purchased from BioLasco (Yi-Lan, Taiwan). The Balb/c mice served as sciatic nerve allograft donors. The C57BL/6 mice were randomized into the group of nerve allograft transplantation with FK506 treatment and the group without FK506 treatment. These species of mice were selected on the basis of disparity at the MHC locus and prior experience with reciprocal rejection of grafts between these murine strains [18]. The mice were anesthetized by intraperitoneal injection of an anesthetic cocktail consisting of 0.1 mg/g ketamine and 0.01 mg/g xylazine. The anesthetized

ORGANISM(S): Mus musculus

SUBMITTER: Ching-Hua Hsieh 

PROVIDER: E-GEOD-60624 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress