Efficient detection and purification of cell populations using synthetic microRNA switches

ABSTRACT: Isolation of specific cell types, including pluripotent stem cell (PSC)-derived populations, is frequently accomplished using cell surface antigens expressed by the cells of interest. However, specific antigens for many cell types have not been identified, making their isolation difficult. Here, we describe an efficient method for purifying cells based on endogenous miRNA activity. We designed synthetic mRNAs encoding a fluorescent protein tagged with sequences targeted by miRNAs expressed by the cells of interest. These miRNA switches control their own translation levels by sensing miRNA activities. Several miRNA switches (miR-1-, miR-208a-, and miR-499a-5p-switches) efficiently purified cardiomyocytes differentiated from human PSCs, and switches encoding the apoptosis inducer Bim enric

ORGANISM(S): Homo sapiens

SUBMITTER: Yoshinori Yoshida 

PROVIDER: E-GEOD-60633 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress