A validated gene regulatory network and GWAS identifies early regulators of T-cell associated diseases (Th1 Th2)

ABSTRACT: In this work we present an analytical strategy to systematically identify early regulators by combining gene regulatory networks (GRN) with GWAS. We hypothesized that early regulators in T-cell associated diseases could be found by defining upstream transcription factors (TFs) in T-cell differentiation. Time series expression and DNA methylation profiling of T-cell differentiation identified several upstream TFs, of which TFs involved in Th1/2 differentiation were most enriched for disease associated SNPs identified by GWAS. Naïve CD4+ T cells were isolated from buffy coat of four healthy donors using a naïve CD4+ T cell isolation kit (Miltenyi, Bergisch-Gladbach, Germany). Naïve CD4+ T cells were stimulated with plate-bound anti-CD3 (500 ng/mL), soluble anti-CD28 (500 ng/mL), in the p

ORGANISM(S): Homo sapiens

SUBMITTER: Danuta Gawel 

PROVIDER: E-GEOD-60678 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress