Deconstructing the dynamic transcriptional program of pluripotent stem cells.

ABSTRACT: Pluripotent stem cells (PSCs) are capable of dynamic interconversion between distinct substates, but the regulatory circuits specifying these states and enabling transitions between them are not well understood. We set out to address this issue and map the landscape of gene expression variability in PSCs by single-cell expression profiling of PSCs under different chemical and genetic perturbations. We find that signaling factors and developmental regulators show highly variable expression in PSCs, with expression states for some variable genes heritable through multiple cell divisions. Expression variability and population heterogeneity can be influenced by perturbation of signaling pathways and chromatin regulators. Strikingly, either removal of mature miRNAs or pharmacologic blockage of

ORGANISM(S): Mus musculus

SUBMITTER: Patrick Cahan 

PROVIDER: E-GEOD-60749 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress