CRISPR reveals a distal murine-specific enhancer required for Sox2 expression in mouse embryonic stem cells

ABSTRACT: The pluripotency of embryonic stem cells (ESCs) is maintained by a small group of master transcription factors including Oct4, Sox2 and Nanog. These core factors form a regulatory circuit controlling the transcription of a number of pluripotency factors including themselves. Although a lot of previous studies have identified key factors regulating this core network in trans, the contribution of cis-regulatory DNA sequences on the transcription of these key pluripotency factors remains elusive. We analyzed epigenomic data within the 1.5 Mb gene-desert regions around Sox2 gene and predicted only one 13kb-long enhancer located 100kb downstream of Sox2 in mouse ES cells. This enhancer is occupied by Oct4, Sox2, Nanog, and mediator complex and forms a long-range DNA looping to Sox2 locus. We hy

ORGANISM(S): Mus musculus

SUBMITTER: Fulai Jin 

PROVIDER: E-GEOD-60763 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress