Redifferentiation of adult human M-NM-2 cells expanded in vitro by inhibition of the WNT pathway

ABSTRACT: In vitro expansion of adult human islet M-NM-2 cells is an attractive solution for the shortage of tissue for cell replacement therapy of type 1 diabetes. Using a lineage tracing approach, we have demonstrated that M-NM-2-cell-derived (BCD) cells rapidly dedifferentiate in culture and can proliferate for up to 16 population doublings. Dedifferentiation is associated with changes resembling epithelial-mesenchymal transition (EMT). The WNT pathway has been shown to induce EMT and plays key roles in regulating replication and differentiation in many cell types. Here we show that BCD cell dedifferentiation is associated with M-NM-2-catenin translocation into the nucleus and activation of the WNT pathway. Inhibition of M-NM-2-catenin expression in expanded BCD cells using short hairpin RNA resu

ORGANISM(S): Homo sapiens

SUBMITTER: Metsada Pasmanik-Chor 

PROVIDER: E-GEOD-60803 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress