Interaction with WDR5 promotes target gene recognition and tumorigenesis by MYC

ABSTRACT: We report a comparison of the genome-wide binding patterns of MYC and WDR5, and the effects of a mutation in MYC (WBM) that disrupt the MYC-WDR5 interaction. The experimental design included two distinct experimental strategies. For comparison of genome-wide binding patters of wild-type (WT) and WDR5 binding-deficient (WBM) MYC, HEK293 cells were engineered by retroviral transduction to express either FLAG-tagged WT-MYC, FLAG-tagged WBM-MYC, or an empty vector control ("vec") ChIP was performed on all samples using the anti-FLAG antibody, and co-precipitating DNAs subject to next-generation sequencing. Samples "2514-WPT-1_1", "2514-WPT-5_1" and "2514-WPT-13_1" are three independent biological replicates of ChIPs performed on the "vec" contr

ORGANISM(S): Homo sapiens

SUBMITTER: Qingguo Wang 

PROVIDER: E-GEOD-60897 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress