Dilution and amplification control study

ABSTRACT: Mouse adult female brainM-bM-^@M-^Ys cortex (C57BL/6, Charles River Laboratories, Inc.) was isolated and stored immediately at -80M-BM-0C. Subsequently, the mRNA (15M-BM-5g) was isolated using TRIzol Reagent and MicroFastTrack 2.0 Kit (Invitrogen). A Sample of 5M-BM-5g was assessed on Affymetrix Mouse 430.2 array. Aliquots from the leftovers of the same cortical mRNA were diluted to single-cell RNA levels (0.1, 1, and 10 pg) and independently aRNA amplified for a total of 2 and 4 rounds and assessed on Affymetrix Mouse 430.2 arrays. A Sample of 5M-BM-5g of mRNA was assessed on Affymetrix Mouse 430.2 array. Aliquots from the leftovers of the same cortical mRNA were diluted to single-cell RNA levels (0.1, 1, and 10 pg) and independently amplified, as described above, for a total of 2 and 4 r

ORGANISM(S): Mus musculus

SUBMITTER: Stephen Fisher 

PROVIDER: E-GEOD-61081 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress