Global regulation of neuronal transcriptome by miR-9/tristetraprolin circuitry

ABSTRACT: Nervous system (NS) development relies on coherent up-regulation of extensive sets of genes in a precise spatiotemporal manner. How such transcriptome-wide effects are orchestrated at the molecular level remains an open question. Here we show that 3’-untranslated regions (3’UTRs) of multiple neuronal transcripts contain A/U-rich cis-elements (AREs) recognized by tristetraprolin (TTP/Zfp36), an RNA-binding protein previously reported to destabilize mRNAs encoding predominantly cytokines, growth factors and proto-oncogenes. We further demonstrate that the efficiency of ARE-dependent mRNA degradation declines during neural differentiation due to a decrease in the TTP protein expression mediated by the NS-enriched microRNA miR-9. Our experiments with transgenenic cell lines suggest that TTP do

ORGANISM(S): Mus musculus

SUBMITTER: Wencheng Li 

PROVIDER: E-GEOD-61098 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress